A lac promoter with a changed distance between -10 and -35 regions

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A lac promoter with a changed distance between -10 and -35 regions.

A lac promotor mutant was constructed by filling in the protruding ends of the HpaII site located within the lac promotor. The mutation, named M42, is a two base pair insertion that changes the distance between the -10 and -35 regions from 18 to 20 residues. The activity of the mutant promotor measured in vivo is 15% of the wild-type promotor. The M42 promotor is sensitive to the catabolite rep...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 1982

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/10.3.903